Listeria monocytogenes biochemical identification kit|BNCC361990 |BNCC

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Biochemical ldentification Kit for listeria monocytogenes

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	3 to 5 single colonies were selected from the plate and mixed with 5mL sterile PBS to make a bacterial suspension. 100 μL of bacterial suspension was inoculated into each microvial flask and test tube (solid medium was directly picked up and inoculated with a single colony). The inoculated vials and test tubes are then plugged with rubber plugs, cultured by semi-plug, standing upright in the groove of the inner support, or placed in a suitable bottle rack, cultured in an incubator at 35-37 ℃ for 24-48h, and the results are interpreted according to "GB4789.30-2016 Food Microbiological Examination Listeria Monocytogenes Examination.
Growth conditions	culture temperature 37 ℃; culture time 18~24 hours; gas environment aerobic;
Storage conditions	2-8 ℃
Safety level	1
application	It is applicable to the GB4789.30-2016 food microbiological examination of Listeria monocytogenes and the physiological and biochemical identification of Listeria monocytogenes.
Sharing mode	Public welfare sharing