BeNa Culture Collection
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| Culture medium | Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃) |
| Subculture procedure | Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 55 ℃ for 40s, and the second to third steps are cycled 40 times; Fluorescence Channel: FAM. |
| Growth conditions | 25 ℃;5-7 days; aerobic; |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| Separation substrate | Root |
| application | Suitable for Fusarium oxysporum nucleic acid detection |
| Sharing mode | Public welfare sharing |
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