BeNa Culture Collection
Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension is diluted to obtain the target concentration, and then subpacked and freeze-dried. |
Growth conditions | 37°C;18-24h; aerobic |
Storage conditions | -80 ℃ |
Safety level | 2 |
morphology | The colony has a diameter of 1-2mm, round, neat edges, opaque, yellow front, raised in the middle, bright surface, smooth surface, moist texture, easy to pick up, G (blue-purple), cocci |
application | Testing Antibacterial Hand Sanitizer Formula Drug Discovery Gut Study |
Sharing mode | Public welfare sharing |