Staphylococcus aureus subspecies Aureus 502A quantitative strain|BNCC351958 |BNCC

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Quantitative strains of staphylococcus aureus subsp. aureus Rosenbach

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic
Growth characteristics	Cells rounded or ovoid, multilaterally budded.
Storage conditions	-80 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, with neat edges, opaque, gray-white front, convex middle, smooth surface, easy to stir up, G + (blue-purple), cocci
application	It is mainly used for medium performance testing, positive control, laboratory ability verification, personnel ability verification, laboratory quality control and method evaluation.
Sharing mode	Public welfare sharing