Aspergillus niger Spore Count Standard Material|BNCC359089 |BNCC

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Reference material of aspergillus niger spores count

Culture medium	Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃)
Subculture procedure	Before use, it was taken out from -20°C and equilibrated at room temperature for 10 minutes, and the lyophilized standard substance was reconstituted by accurately weighing (1.09±0.01)g(20°C) of sterile distilled water with a balance.
Growth conditions	25-28 ℃, strictly aerobic, 5-7d.
Growth characteristics	The colony is white and filamentous, producing a large number of black spores, with yellowish brown on the reverse side. The colony surface is flat, with radial grooves, velvet texture and a small amount of colorless exudate. Conidia head spherical, 50-80μm in diameter; The diameter of the spore stem is 10-15μm, and the wall is smooth. The apical capsule is spherical, with a diameter of 30-40μm, and the surface is fully fertile. The spore-producing structure is double-layered, with a stem base of 10-12 × 2-3μm; Bottle stem 6-8 × 2-3μm; Conidia spherical, with a diameter of 3-4μm and rough wall.
Storage conditions	-20 ℃, protected from light
Safety level	1
application	research; analysis and detection. 2015 edition of Chinese Pharmacopoeia quality control strains, GB 4789.15-2010 mold and yeast count positive control strains. Spore-producing.
Sharing mode	Public welfare sharing