Pseudomonas aeruginosa quantitative strain|BNCC354700 |BNCC

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Quantitative strains of pseudomonas aeruginosa

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic
Growth characteristics	The strain was cultured on nutrient meat juice agar medium at 37 ℃ for 3 days, and the thallus was rod-shaped, 0.61 μm × 1.22~2.13 μm, gram-negative, and did not produce spores.
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 0.5-1mm in diameter, round, with neat edges, opaque, gray-white front, convex in the middle, bright surface, smooth surface, moist texture, easy to pick up, G-(red), bacilli
Separation substrate	Blood culture
application	research; analysis and detection. 2015 edition of chinese pharmacopoeia quality control strains.
Sharing mode	Public welfare sharing