Staphylococcus aureus quantitative strains|BNCC361323 |BNCC

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Quantitative strains of staphylococcus aureus

Culture medium	Tryptone: 17.0, soy peptone: 3.0, sodium chloride: 5.0, dipotassium hydrogen phosphate: 2.5, glucose: 2.5g,pH:7.3±0.2(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃;18-24h; aerobic;
Storage conditions	-20 ℃
Safety level	2
morphology	Round, neat edges, opaque, golden yellow on the front, light color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, no pigment, G + (blue purple), cocci, purity: pure
application	It is mainly used for medium performance testing, positive control, laboratory ability verification, personnel ability verification, laboratory quality control and method evaluation.
Sharing mode	Public welfare sharing