BeNa Culture Collection
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| Culture medium | Bovine brain extract powder: 4.0, bovine heart extract powder: 4.0, peptone: 5.0, casein peptone: 16.0, sodium chloride: 5.0, glucose: 2.0, disodium hydrogen phosphate: 2.5, agar: 13.5,pH:7.4±0.2(25 ℃) |
| Subculture procedure | Brain extract powder: 5.0g, heart extract powder: 12.5g, peptone: 5.0g, tryptone: 10.0g, sodium chloride: 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 15.0g (not included in liquid medium),pH 7.4±0.2. 121 ℃,15min sterilization. |
| Growth conditions | 30 ℃;24-48h; aerobic; |
| Storage conditions | -80 ℃ |
| Safety level | 2 |
| morphology | The colony is 1-2mm in diameter, round, neat edges, opaque, white on the front, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G (blue-purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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