Quantitative strain of Salmonella paratyphi B|BNCC354701 |BNCC

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Quantitative strains of salmonella paratyphi β

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	37 ℃; aerobic
Growth characteristics	Gram-negative bacilli, with a size of 0.6-2.0μm × 1.0-4.0 μm, usually move with peripartum flagella. facultative anaerobic, low nutritional requirements; Citrate cannot be used as the sole carbon source; Gas production, most strains do not produce H2S, ferment arabinose but not xylose, are only pathogenic to humans, and can cause symptoms similar to typhoid fever.
Storage conditions	-20 ℃
Safety level	2
morphology	The colony is 1-2mm in diameter, round, with neat edges, opaque, light color, light color, convex in the middle, smooth surface, bright surface, moist texture, easy to stir up, no pigment, G-(red), bacillus, purity: pure
application	research; analysis and detection. Research, quality control, 2015 edition of chinese pharmacopoeia quality control strains.
Sharing mode	Public welfare sharing