Serratia muculae quantitative strain|BNCC360064 |BNCC

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Quantitative strains of Serratia marcescens

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried;
Growth conditions	30 ℃;18-24h; aerobic;
Growth characteristics	G + cocci, single, paired, irregularly stacked. No sprouts. Ornithine decarboxylase negative, fermentation of glucose, maltose, lactose, sucrose acid production does not produce gas, fermentation of mannitol. Methyl red reaction was positive, V-Pu test was weakly positive. A transparent hemolytic ring was formed around the blood plate colonies. Production of golden yellow pigment. facultative anaerobic, optimum pH7.0-7.5.
Storage conditions	-20 ℃
Safety level	1
morphology	Size: 2-4mm shape: round edge: neat transparency: translucent color: orange bulge degree: middle raised surface: bright and smooth texture: wet
Sharing mode	Public welfare sharing