BeNa Culture Collection
Culture medium | Yeast extract: 3.0, malt extract: 3.0, glucose: 10.0, casein peptone: 5.0, agar: 20.0,pH:6.2±0.2(25 ℃) |
Subculture procedure | ① According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; ④ According to the actual concentration after freeze-drying, the bacterial suspension was diluted to obtain the target concentration, and then subpacked and freeze-dried; |
Growth conditions | 28 ℃;24-48h; aerobic; |
Growth characteristics | Cells rounded or ovoid, multilaterally budded. |
Storage conditions | -20 ℃ |
Safety level | 1 |
morphology | Milky White, raised, moist, sticky, G + cocci |
application | research; analysis and detection. "Disinfection Technical Specification" Bacteria for Sterilization Effect Test of Traditional Chinese Medicine Instruments, "GB 4789.15-2010 Mold and Yeast Count" Positive Control Strains, 2015 Edition of Chinese Pharmacopoeia Quality Control Strains. |
Sharing mode | Public welfare sharing |