BeNa Culture Collection

Rhesus monkey embryonic kidney cells-BNCC
Rhesus monkey embryonic kidney cells-BNCC
  • Rhesus monkey embryonic kidney cells-BNCC
  • Rhesus monkey embryonic kidney cells-BNCC
  • Rhesus monkey embryonic kidney cells-BNCC

Rhesus monkey embryonic kidney cells

  • Price: $344
  • number:BNCC341046
  • Form:
    Epithelial cell-like, spindle-shaped
Basic package DNA extraction
  • Package A:mycoplasma detection assay + 2 vials of frozen cell
  • Package B:mycoplasma detection assay + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Rhesus monkey embryonic kidney cells
Culture medium DMEM-H complete medium: 90% DMEM-H + 10% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ and put it into PE gloves, quickly submerse into a 37 ℃ water bath pot, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1 minute; (2) Add the dissolved cell liquid into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to the T25 flask containing 5-6mL of complete medium and placed in an incubator for culture. Cell subculturing: ① remove the medium, rinse twice with PBS, add 1-2mL pancreatin (0.25%Trypsin +0.02%EDTA); (2) Observe the digestion under the microscope. When the cell edge shrinks and the adherent is loose (a pasteur pipette can be used to suck up some pancreatin blow somewhere in the cell layer, the cell layer can be seen to detach with naked eyes, I .e. digestion is completed, otherwise digestion is continued), and directly suck out pancreatin, add 5-6ml of complete medium, gently blow the cell layer, blow the cell layer off and blow it off. ③ Dispense the cell suspension into a fresh T25 flask as the ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture in an incubator. (4) Pay attention to the change of pH of media and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches 80%-90%.
Growth conditions Culture temperature 37 ℃; Atmosphere 5% CO2 + 95% air;
Growth characteristics Adherent growth
Storage conditions Liquid nitrogen
Safety level 1
morphology Epithelial cell-like, spindle-shaped
Separation substrate Normal kidney
Sharing mode Public welfare sharing

FRhK-4 rhesus monkey embryonic kidney cells

Adherence, epithelial cell-like

Growth:37 ℃,5% CO2 DMEM culture solution

No. 341046

Product format: 2ml frozen vial x 2, or T25 flask x 1

Biosafety level: 1,handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 4h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of lose of cell viability.

Growth conditions:37 ℃,5% co 2,90% DMEM-H + 10% FBS. DMEM-H:DMEM high sugar culture solution, containing glutamine and sodium pyruvate.

Recovery steps:
(1)Prepare a new 100mm culture dish containing 12ml of the above culture medium;
remove the frozen vial from liquid nitrogen or refrigerator at -80℃,  thaw the vial in  37°C water bath for 1-2 minutes. Transfer the vial to biosafety cabinet for culture as soon as the contents are completely thawed.
(2)Draw the solution with a sterile pipette, drip into a new culture dish, and mix it evenly by shaking clockwise;
(3)Put it into incubator (37℃,5%CO2), change the media overnight, and it will grow up in 4-6 days.

Subculture/ cryopreservation:  remove old medium, and rinse twice with PBS, add 2ml of 0.25%Trypsin+0.02%EDTA, do not shake the culture dish during the period. Observed under the microscope, remove most of the trypsin(with 0.5ml left) when the cells detach. transfer it to the incubator for digestion about 1minute, and take it out.

(1)Subculture: terminate digestion with 6ml  culture media, aspirate and passage of 1:2

(2) Cryopreservation: terminate digestion with 3ml of cryopreservation solution (50%basic medium+40%FBS+10%DMSO), aspirate and dispense into 3 frozen vials, and cryopreserve at -80 ℃ with a programmed cooling box.

Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard recovery record
viability adherence is observed in 8hours,  the cell adherence rate ≥ 80.0% in 100hours adherence is observed in 18 hours,  the cell adherence rate ≥ 80.0% in 96hours
cell morphology adherent, epithelial cell-like adherent, epithelial cell-like, spindle-shaped
attached figure
Conclusion good viability, no abnormal cell morphology, qualified
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