BeNa Culture Collection

Mouse mammary tumor cells-BNCC
Mouse mammary tumor cells-BNCC
  • Mouse mammary tumor cells-BNCC
  • Mouse mammary tumor cells-BNCC
  • Mouse mammary tumor cells-BNCC
【C127】

Mouse mammary tumor cells

  • Price: $373
  • number:BNCC354618
  • Form:
    Adherent, epithelial cell-like, polygonal
Basic package DNA extraction
Package:
  • Package A:mycoplasma detection assay + 2 vials of frozen cell
  • Package B:mycoplasma detection assay + 2 vials of frozen cell + 100ml of complete medium
Essential Information Certificate Related Products
Mouse mammary tumor cells
Culture medium DMEM-H complete medium (including 10% FBS):90% DMEM-H + 10% FBS
Subculture procedure Recovery steps: ① Take out the frozen vial from liquid nitrogen or -80 ℃ and put it into PE gloves, quickly submerse into a 37 ℃ water bath pot, shake the frozen vial to accelerate dissolution, and it is advisable to dissolve all within 1 minute; (2) Add the dissolved cell liquid into a centrifuge tube containing 9ml of complete culture medium in an ultra-clean table, centrifuge at 1000-1200rpm/min for 5 minutes, discard the supernatant, resuspend the cells with 1-2ml of complete media. ③The cell suspension was then added to the T25 flask containing 5-6mL of complete medium and placed in an incubator for culture. cell subculture: ① remove the medium, rinse twice with PBS, add 1-2mL pancreatin (0.25%Trypsin +0.02%EDTA); (2) Observe the digestion situation under the microscope. When the cell edge shrinks and the adherent is loose (a pipette can be used to suck up some pancreatin and gently blow somewhere in the cell layer, and the cell layer can be seen to detach with naked eyes, I .e. digestion, add 5-6ml of complete medium, gently blow the cell layer, blow the cell layer off and blow it off. ③ Dispense the cell suspension into a fresh T25 flask as the ratio of 1:2, add appropriate complete culture medium, mix the cell suspension evenly, and culture in an incubator. (4) Pay attention to the change of pH of media and cell density, renew the medium regularly (2-3 times a week), and repeat the subculture or cryopreservation when the cell density reaches 80%-90%.
Growth conditions Culture temperature 37 ℃; Atmosphere 5% CO2 + 95% air;
Growth characteristics Adherent growth
Storage conditions Liquid nitrogen
Type Adherent growth
Safety level 1
morphology Adherent, epithelial cell-like, polygonal
Separation substrate C- 127 is an untransformed clonal strain derived from RIII mouse breast cancer.
Sharing mode Public welfare sharing

C127 mouse breast tumor cells

adherent, epithelial cell-like

No. 354618

Product format :  2ml frozen vial x 2, or T25 flask x 1

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 4h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of loss of cell viability.

Growth conditions:37 ℃,5% co 2,90% DMEM-H + 10% FBS. DMEM-H:DMEM high sugar culture solution, containing glutamine and sodium pyruvate.

Recovery steps:① 1 new 100mm plate containing 12mL of the above culture solution; (2) the frozen storage tube is taken out of liquid nitrogen or -80 ℃, bathed in water at 37 ℃ for 1~2min, and moved into Recovery insafety cabinet as soon as possible after complete dissolution;③ use sterile straw to suck the dissolved liquid into the new plate and shake well clockwise. ④ Put it into (37 ℃,5% CO2) incubator, change the liquid overnight, and fill it in 4-6 days.

Subculture/cryopreservation: Remove the medium, rinse twice with PBS , and add 1-2mL pancreatin (0.25% Trypsin + 0.02% EDTA);  observe under a microscope, do not shake the culture dish during this period, when the cells detach, suck out most of the pancreatin with about 0.5ml remained, move to the incubator for digestion, and take out in about 2min. For subculture, digestion is terminated by 6mL of culture solution, and subculture at 1:2. For cryopreservation, terminate the digestion with 3mL of cryopreservation solution ( 50% base medium+40%FBS+10%DMSO ), blow evenly, dispense into 3 frozen vials, and freeze at -80 ℃ with a program cooling box.

Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

Item quality standard Recovery record
viability: adherence is observed in 18 hours, the cell adherence rate ≥ 80.0% in 120 hours adherence is observed in 18 hours, cell adherence rate ≥ 80.0% in 96 hours
cell morphology: Adherence, epithelial cell-like Adherent, epithelial-like, polygonal
attached:
Conclusion: good viability, and no abnormal cell morphology, qualified
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