BeNa Culture Collection
Culture medium | LB + 50mcg/ml ampicillin: yeast extract 5.0g, peptone 10.0g,NaCl 10.0g, distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. After sterilization, ampicillin was added when the culture medium was cooled to 40-50 ℃ with a concentration of 50mcg/ml. |
Growth conditions | 37 ℃;18-24 hours; Aerobic; |
Storage conditions | 2-8 ℃ |
application | pFA6a-kanMX6 plasmid extraction |
Sharing mode | Public welfare sharing |