Bovine viral diarrhea virus 1 RNA standard|BNCC364957 |BNCC

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Bovine viral diarrhea virus 1 RNA standard

Culture medium	90%DMEM-H+10%HS
Subculture procedure	When using this standard, it is moved to room temperature (20~25 ℃) and then thawed, shaken evenly, centrifuged briefly, and placed on ice in a low temperature environment for temporary storage for use, trying to avoid repeated freezing and thawing.
Growth conditions	Culture temperature 37 ℃; Culture time 2-6 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
Separation substrate	The National Animal Disease Laboratory seals the spleens of naturally occurring BVD death cases in cattle.
application	The standard, not infectious, no biological safety risk, suitable for real-time fluorescence quantitative PCR and other methods of bovine viral diarrhea virus 1 nucleic acid qualitative and quantitative determination, as well as laboratory quality control and kit development and evaluation.
Sharing mode	Public welfare sharing