Human rhinovirus 77 RNA lab quality control|BNCC372352 |BNCC

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Human rhinovirus 77 RNA lab quality control

Culture medium	EMEM complete medium (with 2% FBS):EMEM + 2% FBS
Subculture procedure	Remove from the refrigerator, melt completely, vortex and mix well, ready to use. Note: The quality control is inactivated particles, which need to be used after nucleic acid extraction. The extraction or purification reagents should be prepared by themselves, and repeated freezing and thawing should be avoided before extraction. The minimum sampling volume is 200 μl.
Growth conditions	Culture temperature 33 ℃; Culture time 1-7 days; Gas environment 5% CO2 + 95% air;
Storage conditions	-80 ℃
Safety level	2
application	The quality control product is developed by Henan engineering center of industrial microbial strain, which is an inactivated virus suspension of human rhinovirus 77, and can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc. by digital PCR.
Sharing mode	Public welfare sharing