BeNa Culture Collection
Subculture procedure | Sample processing: sample processing shall be carried out according to GB/T 4789.5-2003 Shigella Detection in Food; Experimental preparation: the various components in the kit are used to dissolve on ice, slightly mixed and briefly centrifuged, and then placed on ice for later use; Reagent preparation: experimental reaction system (25 μL),qPCR mixed reaction solution 15 μL, template 1 μL, sterile ddH2O 9 μL (when the diluted bacteria solution is used as a template to add 10 μL); Reaction conditions: 95 ℃ 1min 1cycle;95 ℃ 10s, 52 ℃ 30s 40cycle (when diluted bacteria liquid is used as template for the first step reaction for 3min); Fluorescence channel: FAM; |
Storage conditions | -20 ℃; Keep away from light |
Safety level | 0 |
application | It is suitable for the auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples. |
Sharing mode | Public welfare sharing |
Shigella nucleic acid test kit certificate
1. Product information
Sample name: Shigella nucleic acid detection kit (fluorescent PCR method)
Sample number: 361199
Sample batch: 220402
2. Product features
Reagent composition | reaction solution 375μL/tube, sterile ddH2O 1mL/tube, positive quality control 25μL/tube, negative quality control 25μL/tube |
traceability | Shigella ATCC12022, Escherichia coli ATCC25922 |
save and transport | -20 ℃, valid for 1 year, dry ice transportation |
applicable fields | Suitable for auxiliary diagnosis of Shigella infection in water-like feces, suspected contaminated water, food and other samples |
*Note: If you have any questions about this kit, please contact our center (BNCC) for help before use |
3. Instructions
Sample processing: sample processing according to《GB/T 4789.5-2003 Shigella in Food》;
Experiment preparation: Dissolve the various components in the kit on ice before use, mix gently and centrifuge briefly, then place on ice for later use;
Reagent preparation: experimental reaction system (25 μL), 15 μL qPCR mixed reaction solution, 1 μL template, 9 μL sterile ddH2O (10 μL when using diluted bacterial solution as template);
Reaction conditions: 95°C 1min 1cycle; 95°C 10s, 52°C 30s 40cycles (when the diluted bacterial solution is used as the template for the first step reaction for 3min);
Fluorescence channel: FAM;
4. Precautions
Negative: The sample has no exponential growth curve, or has a lower amplification curve, and the Ct value is >35;
Positive: The sample has a typical exponential growth curve, and the Ct value is ≤35;
5. Preservation and transportation
1. Dissolve the various components in the kit on ice before use, mix thoroughly and centrifuge briefly and place on ice for later use;
2. In order to ensure the sensitivity of the detection, it is recommended that the qPCR mixed reaction solution be frozen and thawed no more than 3 times;
3. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;
Henan Engineering Research Center of Industrial Microbiology
Website: www.bncc .org .cn Tel: 400-6699-833