Lactobacillus frivolum nucleic acid reference material (Heat inactivated) (Strongly positive)|BNCC360609 |BNCC

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Lactobacillus frivolum nucleic acid reference material (Heat inactivated) (Strongly positive)

Culture medium	MRS medium (English name: MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	Before use, balance the reference at room temperature for 30min and thaw. Equilibrium to room temperature, vortex oscillation and mixing, according to the nucleic acid extraction reagent instructions to extract nucleic acid after use, nucleic acid extraction or purification reagents need to be self-provided.
Storage conditions	-80 ℃, kept away from light
Safety level	2
application	This product is a heat-inactivated strong positive reference for the evaluation of Lactobacillus curl nucleic acid detection reagent, which can be used for method establishment, work standard assignment, method confirmation and evaluation, product quality control, etc.
Sharing mode	Public welfare sharing

Lactobacillus crispatus nucleic acid reference certificate

1. Product information

Sample name: Lactobacillus crispatus nucleic acid reference (heat inactivated)

Sample number: 360609

sample batch: 220107

2.Product features

Description	Appearance is transparent liquid, packaged in 1.5mL cryovial
Traceability	CGMCC1.2743 Lactobacillus curl
Concentration value	≥10^6CFU/mL
Uniformity and stability	Random sampling, the uniformity of the results is good; long-term stability test, the stability is qualified
Applicable method	Related research for pathogen nucleic acid detection reagents
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, the reference material was equilibrated at room temperature for 30 minutes and thawed. Equilibrate to room temperature. After vortexing and mixing, extract the nucleic acid according to the instructions of the nucleic acid extraction reagent before use. The nucleic acid extraction or purification reagent should be prepared by yourself.

4. Notes

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt;

2. This material is inactivated by heat inactivation, which is likely to cause damage to protein antigens, and is not suitable for experiments related to protein antigens;

3. The research materials should be used as soon as possible after freezing and thawing, and can be stored at -80°C and below, but avoid repeated freezing and thawing more than 3 times;

4. During the use of this research material, it should be treated as infectious substances. Please do not touch the samples in the bottle directly with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them;

5. Preservation and transportation

long-term storage:-80 ℃, valid for 2 years; Transportation conditions: dry ice transportation, the time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833