Quality control sample of total bacterial count in drinking water|BNCC360334 |BNCC

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Quality control sample of total bacterial count in drinking water

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	After taking out the quality control sample from the refrigerator at -20 ℃, placing it at room temperature for 30 min, then opening it in the biological safety cabinet, immediately adding 5mL of sterile water or sterile physiological saline to re-dissolve the freeze-dried quality control product, and fully mixing it to prepare a sample suspension, which is a quality control sample with a concentration of 3000~9000CFU/mL.
Growth conditions	37°C, aerobic, 18-24h;
Storage conditions	-20 ℃, cold storage away from light; Ice bag transportation
Safety level	2
application	GB/T 5750.12-2006 Standard Test Method for Drinking Water Microbial Indicators Total Colony Count Method "HJ 1000-2018 Determination of Total Bacteria in Water Quality Plate Counting Method" The number of strains required for production is: BNCC269342, BNCC286903, BNCC238481 and BNCC293727.
Sharing mode	Public welfare sharing

Quality control sample certificate for total number of colonies in drinking water

1. Product information

Sample name: Quality control sample of total bacterial count in drinking water

Sample number: 360334

Sample batch: 220531

2. Product features

Description	Appearance is white powder (lyophilized powder), containing protective agent, packaged in 7mL vial
Traceability	ATCC 25922 Escherichia coli, ATCC 13048 Enterobacter aerogenes ATCC 6633 Bacillus subtilis, ATCC 6538 Staphylococcus aureus
Reference value	8.2x10³CFU/mL
Reference Range	3x10³~9x10³CFU/mL
Uniformity and stability	Random sampling, the results are uniform; long-term stability test, the stability is qualified
Applicable method	《GB/T 5750.12-2006 Standard Test Methods for Drinking Water-Microbial Indicators Plate Counting Method for Total Colonies》《HJ 1000-2018 Water Quality Determination of Total Bacteria Plate Counting Method》
*Note: If you have any questions about the quality control samples, please contact our center (BNCC) for help before use

3.Instructions:

After the quality control samples were taken out of the -20°C refrigerator, they were placed at room temperature for 30 minutes to equilibrate, and then opened in a biological safety cabinet. Immediately add 5 mL of sterile water or sterile normal saline to reconstitute the lyophilized quality control products, and mix thoroughly. After homogenization, prepare a sample suspension, which is the quality control sample, with a concentration of 3x10³~9x10³CFU/mL.

4. Notes:

1. After receiving the goods, please keep them properly. If there is any damage, please contact the center within 24 hours after receipt.

2. Please operate in a sterile environment and in a biological safety cabinet. After opening, you must use it all at once.

3. This product contains pathogenic microorganisms. Please do not directly touch the samples in the bottle with your hands. After use, please sterilize the residual samples at high temperature and high pressure and discard them.

5. Storage and transport:

long-term storage: -20 ℃, valid for 1 year; Transportation conditions: ice bag transportation, the time should not exceed 7 days.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc .org .cn ; Tel: 400-6699-833