BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Growth conditions | 37 ℃;18-24h; Aerobic; |
Storage conditions | -80 ℃ |
Separation substrate | Clinical isolates |
application | Sensitive test; Antibacterial test; Food testing; Cosmetic testing; Culture control; Quality control strain; API verification bacteria, GB 4789.38-2012 Escherichia coli count positive control strain. |
Sharing mode | Public welfare sharing |
Escherichia coli
Strain number:BNCC 354945 / ATCC25922
Name: Escherichia coli
Product format: quantitative bacteria tablets Number of viable bacteria: 300 ~ 500CFU/bottle
Product batch: 21122001 Valid until: 2022.12.19
Inspection date: 2021. 12.21 Sampling ratio: ≥2%
Report number: RS21354945- 1 Biosafety level: 1, handle in ultra-clear table or safety cabinet
Item | Inspection description | results |
stain microscopy | gram negative and positive, cell morphology observed under microscope | G-bacilli |
colony morphology | observation of single colony morphology | pale yellow, raised, bright |
Purity | non-selective medium culture, colony observation and microscopic examination | No contaminated bacterial |
Reference value of live bacteria | Non-selective medium plate, viable count | 380CFU/bottle |
Phenotypic and Genotypic Analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | After the BNCC quality control inspection system quality inspection, the product is qualified |
Product list: 300 ~ 500CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;
Validity period: unopened, below -20 ℃, 12 months;
Usage:
1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;
2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;
3. After fully dissolving, it is 300 ~ 500CFU/bottle, which can be sucked in proportion according to the test requirements;
4. Please use up immediately after opening, and do not store it;
Notes:
① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.
② Used bacteria-carrying consumables, please autoclave and discard them.