BeNa Culture Collection

Aspergillus oryzae (Ahlburg) Cohn-BNCC
Aspergillus oryzae (Ahlburg) Cohn-BNCC
Aspergillus oryzae (Ahlburg) Cohn-BNCC
  • BNCC
  • Aspergillus oryzae (Ahlburg) Cohn-BNCC
  • Aspergillus oryzae (Ahlburg) Cohn-BNCC
  • Aspergillus oryzae (Ahlburg) Cohn-BNCC

Aspergillus oryzae (Ahlburg) Cohn

  • Price: $ 86
  • number:BNCC338380
  • Form:
    Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the integrated PDA medium, later producing yellow-green spores, spreading and growing to the edge of the plate, and the back of the medium was yellow.
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Aspergillus oryzae (Ahlburg) Cohn
Culture medium Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure (1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate  evenly. (5) put the plates under the growth conditions.
Growth conditions 28 ℃;5-7 days; Aerobic
Storage conditions 2-8 ℃
morphology Small filamentous fungi, with obvious colonies, white, dense and vigorous hyphae on the integrated PDA medium, later producing yellow-green spores, spreading and growing to the edge of the plate, and the back of the medium was yellow.
application Research; Production. Production of saccharifying enzyme, amylase, alcohol production of saccharifying bacteria.
Sharing mode Public welfare sharing

Description

1. Name:Aspergillus oryzae (Ahlburg) Cohn

2. BNCC No.: 338380

3. Biosafety level: 4

Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

Growth Conditions

1, Czapek ' Sagar: sucrose 30.0g,NNaNO3 3.0 g,MgSO4·7H2O 0.5 g,KCl 0.5g,FeSO4·4H2O 0.01 g,K2HPO4 1.0 g, agar 15.0g, distilled water 1.0 L,pH 6.0-6.5,121 ℃,15min.

2. Atmosphere:aerobic

3, Temperature: 25-28 ℃

Notes:

1.Normal culturing time, 1-2  days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and long-term storage is not recommended.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Users should ensure the safe storage and operation of the strain.Waste generated from the handling process should be discarded after high-pressure sterilization

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