Fusarium solani|BNCC337554 |BNCC

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Fusarium solani

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃;5-7 days; Aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on the integrated PDA medium, white and dense colonies, spreading to concentric circles on the edge of the plate, and yellow on the back of the medium.
Separation substrate	Apple rotten root
application	solanum pathogen
Sharing mode	Public welfare sharing

Fusarium solani

storage conditions: 2~8 ℃

number: 337554

product format: agar slant in 14mm test tube

Validity: 30 days

Biosafety level: 1, handle in ultra-clean table or safety cabinet

Receiving notice:if any abnormality is found on the day of receiving goods, please contact customer service within 24 hours. if the time is overdue, it will be regarded as good receiving goods. The agar slant can be used directly. in principle, it can be used many times without contamination within the storage period, but its vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement service will not be provided if the strain is abnormal and inactivated.

culture conditions: 25-28 ℃, aerobic, integrated PDA,5d. Comprehensive PDA: potato boil 1.0L, glucose 20.0g,KH₂PO₄3.0g,MgSO₄·7H₂O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0 ±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.

Recovery steps:

1 prepare 1-2 pieces of PDA plates;

2 sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet;

3 cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2;

4 lay flat the small pieces to the center of the agar plate;

5 put the plates under the above culture conditions, and the strains can be used when they grow.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viability:	good viability. in 5 days, plate colony is obvious
colony morphology:	Small filamentous fungi have obvious colonies on the integrated PDA medium, white and dense colonies, spreading and growing concentrically to the edge of the plate, and the back of the medium is yellow.
Conclusion:	good viability, no abnormal colony morphology, qualified