BeNa Culture Collection

Neisseria gonorrhoeae-BNCC
Neisseria gonorrhoeae-BNCC
  • BNCC
  • Neisseria gonorrhoeae-BNCC
  • Neisseria gonorrhoeae-BNCC

Neisseria gonorrhoeae

  • Price: $ 229
  • number:BNCC337543
  • Form:
    Purity: pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
Essential Information Certificate Related Products
Neisseria gonorrhoeae
Culture medium Columbia agar chocolate plate (CA-C): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast leaching powder 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 80-90 ℃, then add 5% sterile defibrated sheep blood, shake well and pour the plate to cool for later use.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) open it in the biosafety cabinet, heat the tip of ampoule in the flame, quickly drop sterile water to creak it, and break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃;24-48 hours; 5% CO2
Storage conditions 2-8 ℃
morphology Purity: pure
application Quality control strains; Quality control strains of BBL; Research on sexually transmitted diseases; Mediatesting
Sharing mode Public welfare sharing

Neisseria gonorrhoeae

Storage conditions: 2~8 ℃

No. 337543

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and lose of viability.

Growth conditions:37 ℃,5% CO2, Columbia blood plate (ready-to-use type),24-48h.

Recovery steps:

(1)Prepare 1-2 of above mentioned plates;

(2)Open the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

item test result
viability good viability, in 24-48h strain layer is obvious and colony is typical
colony morphology: (above)

size: 2-4mm shape: round edge: neat transparency: opaque

color: white uplift: middle convex surface: bright and smooth texture: moist and viscous

conclusion good viability, no abnormal colony morphology, qualified
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