Streptococcus agalactiae|BNCC337481 |BNCC

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Streptococcus agalactiae

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilizing the surface of the plate, open it in the biosafety cabinet; (3) suck 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, transfer it into a fresh plate, and spread it evenly; (5) Place the plate under the above culture conditions and culture
Growth conditions	37 ℃;24-48h; Aerobic
Growth characteristics	Non-hemolytic.
Storage conditions	2-8 ℃
morphology	Size: 2-4mm Shape: Round Edge: Irregular Transparency: Opaque Color: Milky White Protuberance: Middle Protuberance Surface: Bright and Smooth Texture: Wet and Viscous
Separation substrate	Milk
application	Quality control strain
Sharing mode	Public welfare sharing

Streptococcus agalactiae

Storage conditions : 2~8 ℃

No. : 337481

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, Colombian blood plate (ready-to-use type),24-48h.

Recovery steps:

(1)Prepare 2 of above mentioned plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water(keep in an anaerobic environment for 24 hours) into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability	good viability,in 48h liquid medium become turbid, obvious strain layer occurs on the plate,colony is typical on marked plate.
colony morphology: (above)	size: 2-4mm shape: round edge: irregular transparency: opaque color: milky white uplift: middle raised surface: bright and smooth texture: moist and viscous
conclusion	good viability, no abnormal colony morphology, qualified