Bacteroides vulgatus|BNCC337471 |BNCC

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Bacteroides vulgatus

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of the plate and open it in biosafety cabinet; (3) Absorb 1-3ml of sterile water into the plate, scrape the bacterial lawn back and forth on the surface of the plate with a sterile spreader to make a bacterial suspension; (4) suck the bacteria suspension with a pasteur pipette, dispense it into a fresh plate evenly. (5) put the plates under the growth conditions.
Growth conditions	37 ℃;48-72h; Anaerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Protuberance: Flat Surface: Bright and Smooth Texture: Wet and Easy to Stir
Separation substrate	Human feces
Sharing mode	Public welfare sharing

Bacteroides vulgatus

Storage conditions: 2~8 ℃

No.: 337471

Product format: freeze dried, 200ul

Validity period: 6 years

Biosafety level: 2, handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally.Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, 48-72h,① Columbia blood plate (ready-to-use type) ② liquid thioglycolate culture medium: casepone (trypsin hydrolysis) 15.0g, yeast extract 5.0g, glucose 5.0g, sodium thioglycolate 0.5g,L-cystine 0.5g, sodium chloride 2.5g, azurite 0.001g, agar 0.75g, distilled water 1.0L,pH 7.1± 0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1)Prepare sterile test tube of 5-10ml liquid medium and 2 plates; Silica gel plug is used for liquid test tube culture medium to ensure ventilation and facilitate deoxygenation. Please put the liquid medium and plate in an anaerobic environment for 24h before usage.

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test result
Viabiility:	good viability. in 48-72h liquid medium become turbid, obvious strain layer occurs on the plate.
colony morphology: (above)	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque color: white uplift: middle convex surface: bright and smooth texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified

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