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Issatchenkia orientalis
Storage conditions : 2~8 ℃
No. : 337336
Product format : freeze dried, 200ul
Validity period : freeze-dried tube 24 months
Biosafety level : 1 , handle in ultra-clean table or safety cabinet
Receiving notice if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.
Growth conditions :25-28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.
Recovery steps:
(1)Prepare 1-2 of YM plates;
(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the YM plates well in 200ul/plate;
(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows: