BeNa Culture Collection

Issatchenkia orientalis-BNCC
Issatchenkia orientalis-BNCC
Issatchenkia orientalis-BNCC
  • BNCC
  • Issatchenkia orientalis-BNCC
  • Issatchenkia orientalis-BNCC
  • Issatchenkia orientalis-BNCC

Issatchenkia orientalis

  • Price: $ 86
  • number:BNCC337336
  • Form:
    Size: general color: milky white shape: round edge: neat edge dry and wet: slightly dry and smooth: slightly rough transparency: opaque uplift: convex
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Issatchenkia orientalis
Culture medium YM medium (YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 28 ℃;24-48h; Aerobic
Storage conditions 2-8 ℃
morphology Size: general color: milky white shape: round edge: neat edge dry and wet: slightly dry and smooth: slightly rough transparency: opaque uplift: convex
Separation substrate Sputum in patients with bronchial mycosis, Colombo, Sri Lanka
application Quality control strain
Sharing mode Public welfare sharing

Issatchenkia orientalis

Storage conditions : 2~8 ℃

No. : 337336

Product format : freeze dried, 200ul

Validity period : freeze-dried tube 24 months

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

Receiving notice if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :25-28 ℃, aerobic, YM medium. YM medium: yeast extract, 3.0g malt extract, 3.0g glucose, 10.0g protein block, 5.0g agar, 20.0g distilled water 1000 ml ,pH 6.2 +/- 0.2,121 ℃,15min.

Recovery steps:

(1)Prepare 1-2 of YM plates;

(2)Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the YM plates well in 200ul/plate;

(4)Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

                                       

item test result
viability good viability, in 40h YM strain layer is obvious
colony morphology size: general color: milky white shape: round edge: neat and wet edges: slightly dry and smooth: slightly rough transparency: opaque protuberance: bulge
conclusion good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified
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