Listeria innocua|BNCC336912 |BNCC

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Listeria innocua

Culture medium	Brain heart extract medium (BHIA/BHIB): brain extract 5.0g, heart extract 12.5g, peptone 5.0g, tryptone 10.0g, sodium chloride 5.0g, glucose 2.0g, disodium hydrogen phosphate 2.5g, agar 20.0g (not included in liquid medium),pH 7.4±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;18-24h; Aerobic
Growth characteristics	Antigenic characteristics: serotype 6a
Storage conditions	2-8 ℃
Separation substrate	bovine brain
application	Type strain. Classification; Research; Culture medium quality control, Listeria detection quality control strains
Sharing mode	Public welfare sharing

1.Description

1.Name: Listeria innocua

2.BNCC No.:336912

3. Biosafety level: 4

2.Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3.Growth Conditions

1. BHIA: 4.0g of bovine brain extract, 4.0g of bovine heart extract, 5.0g of peptone, 16.0g of casein peptone, 5.0g of NaCl, 2.0g of glucose, 2.5g of disodium hydrogen phosphate, 20.0g of agar, pH7.4 ± 0.2. 121 ℃,15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4.Notes:

1.Normal culturing time, 1-2days for bacterial, 3 days for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization