Malassezia furfur (Robin) Baillon|BNCC324536 |BNCC

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Malassezia furfur (Robin) Baillon

Culture medium	Pityrosporum culture medium (MFM): wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0L. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
morphology	The colony diameter is 1-2mm, round, with neat edges, opaque, white on the front, golden yellow on the back, convex in the middle, rough surface, moist texture, easy to pick up, no pigment, G + (blue-purple), cocci, purity: pure
application	Biomedical Research and Development Material
Sharing mode	Public welfare sharing

Malassezia furfur (Robin) Baillon

Storage conditions : 2~8 ℃

No. : 324536

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 2 , handle in safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Culture conditions :30 ℃, aerobic, Pityrosporum medium, 24-48h. Pityrosporum culture medium: wort agar 60.0g, bovine bile (dry) 20.0g, Tween 40 10.0g,Glycerol mono-oleate 2.5g, distilled water 1.0 L. Sterilization at 121 ℃ for 15min.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 24-48hours.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability, in 24-48h strain layer become obvious, colony is typical
colony morphology: (above)	Size: 2-4mm Shape: Round Edge: Neat Transparency: Opaque Color: milky white uplift: middle convex surface: bright and smooth texture: moist and viscous
Conclusion:	good viability, no abnormal colony morphology, qualified