Fusobacterium nucleatum subsp.‖polymorphum (Knorr) Dzink et al.|BNCC311817 |BNCC

Essential Information Certificate Related Products

Fusobacterium nucleatum subsp.‖polymorphum (Knorr) Dzink et al.

Culture medium	Liquid thioglycolate medium (FT): 15.0g of caseptone (trypsin hydrolysis), 5.0g of yeast extract, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 2.5g of sodium chloride, 0.001g of azure, 0.75g of agar, 1.0L of distilled water and 7.1±0.2 of pH value. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;4-5 days; Anaerobic;
Storage conditions	2-8 ℃
morphology	Fusobacterium nucleatum polymorphic subspecies, gram-negative non-spore anaerobic bacillus, slender shape, sharp at both ends, thioglycolate solution Body culture medium culture bacteria liquid is turbid, it is recommended to use liquid culture.
Separation substrate	Inflamed gingiva, adult male
Sharing mode	Public welfare sharing

Fusobacterium nucleatum subsp.polymorphum

No. 311817

Product format: freeze dried,200ul

Storage conditions: 2~8 ℃

Validity period: Freeze-dried tube for 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Recovery/subculture:37 ℃, anaerobic, Columbia blood plate (finished product).

Recovery steps:

(1) Prepare 1-2 of above mentioned plates;

(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;

(4) Put the plates under the above culture conditions for cultivation for 24-48 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability	good viability，in 72 h, Colombian blood plate colony is obvious
colony morphology: (above)	size: small color: off-white shape: round edge: neat edge wet and dry: wet and smooth: smooth transparency: opaque uplift: uplift
Conclusion	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified