Aspergillus flavus|BNCC261205 |BNCC

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Aspergillus flavus

Culture medium	Potato Glucose Agar (PDA): Potato Boiled Solution 1.0L, Glucose 20.0g, Agar 15.0g, Natural pH. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of sterile water into a freeze dried ampoule, fully rehydrate, inoculate a plate with the solution at 200ul/plate; (4) Put the plate under the above culture conditions for 5-7 days.
Growth conditions	28 ℃,5-7 days, aerobic
Storage conditions	2-8 ℃
morphology	Small filamentous fungi, with obvious colonies on PDA medium, white hyphae at the initial stage, yellow spores at the later stage, and light on the back of the medium Yellow.
Separation substrate	human biopsy United States
Sharing mode	Public welfare sharing

Aspergillus flavus Link

Storage conditions: 2~8 ℃

No. : 261205

Product format: freeze dried, 200ul

Validity : 6 years

Biosafety level: 1 , handle in ultra-clean table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The tube bevel can be used directly. In principle, it can be used many times without stain during the storage period, but the vitality will gradually decrease with time. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :28 ℃, aerobic, PDA,5-7 days. PDA: potato boiling solution 1.0L, glucose 20.0g, agar 15.0g (without liquid medium), natural pH. Sterilization at 121 ℃ for 15min. Potato boiling solution: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect filtrate to a constant volume of 1.0L.

Recovery steps:
(1) Prepare 1-2 of above mentioned plates;
(2) Open it in the biosafety cabinet, sterilize of the ampoule, heat the tip in a flame, quickly drop sterile water to creak it, then break it with forceps;
(3) Draw 0.5ml of sterile water into the freeze dried ampoule, make it fully dissolved, and distribute the solution to the plates well in 200ul/plate;
(4) Put the plates under the above culture conditions for cultivation for 3-5 days.
Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability,in 7 days, strain layer is obvious
colony morphology:	Small filamentous fungi with obvious colonies on PDA medium, Early white hyphae, late yellow spores, pale yellow on the back of the medium
Conclusion:	good viability, no abnormal colony morphology, qualified ;