Sphingobacterium sp.|BNCC194789 |BNCC

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Sphingobacterium sp.

Culture medium	Columbia blood plate (CA-B): casein pancreatin digest 10.0g, cardiopancreatin digest 3.0g, corn starch 1.0g, meat gastric enzyme digest 5.0g, yeast extract 5.0g, sodium chloride 5.0, agar 20.0g, distilled water 1.0L,pH 7.3±0.2. After sterilization at 121 ℃ for 15min, cool to 55 ℃, add 5% sterile defibrated sheep blood, shake well, pour the plate and cool it for later use.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	30 ℃;18-24h; Aerobic
Storage conditions	2-8 ℃
morphology	Size: 1-2mm Shape: Round Edge: Neat Transparency: Opaque Color: off-white bulge: middle bulge surface: bright and smooth texture: moist and easy to stir up
Separation substrate	Raw milk
application	Can lead to denaturation of dairy protein
Sharing mode	Public welfare sharing

Sphingobacterium sp.

No. 194789

Storage conditions: 2~8 ℃

Product format： freeze dried, 200ul or flat plate 1

Validity period: Freeze-dried tube 24 months, flat plate 7 days

Biosafety level: 1, handle in ultra-clean table or biosafety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:30 ℃, aerobic, Colombian blood plate (finished product).

Recovery steps:

(1)Prepare 1-2 pieces of Blood agar plates;

(2)Sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3)Draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

(4)Put the agar plates under the above culture conditions for cultivation 18-24 hours.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

item	test results
viability:	good viability, in 20h Colombian blood plate colony is obvious
colony morphology:	size: medium color: pink orange shape: round edge: neat edge wet and dry: wet and smooth: smooth and bright transparency: opaque bulge: bulge
conclusion:	good viability, and colony morphology is not abnormal, completely consistent with the above figure, qualified

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