Lactobacillus helveticus|BNCC187926 |BNCC

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Lactobacillus helveticus

Culture medium	MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;24-48h; Aerobic;
Storage conditions	2-8 ℃
Type	G+
morphology	G +, the thallus is long rod-shaped, the ends are blunt and round, arranged in a chain shape, without spores, and with different stained grains. The colony is not round, the surface is rough, flat, the edge is uneven, light yellow, opaque.
application	Produces yogurt. Fermented glucose, lactose, non-fermenting xylose, fructose, sucrose, arabinose. Acid production from gluconate, negative contact enzyme, weak ammonia production from arginine, acid production from milk reaction, coagulation, reduction, facultative anaerobic. Growth at 45 ℃. Production of lactic acid, "GB 4789.28 medium and reagent quality requirements standard strain recommendation"
Sharing mode	Public welfare sharing

Lactobacillus helveticus

Storage conditions: 2~8 ℃

No.: 187926

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level:1

Receiving notice: if any abnormal situation such as damage is found on the day of receipt, please contact customer service within 24 hours. Dry powder cannot be retained once used up. please refer to the attached page for details of the use of bacterial liquid. Please operate in strict accordance with this instruction,otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, aerobic, MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0l. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

1. Prepare one sterile test tube liquid culture medium, 25mL of 18mm large test tube liquid, and plug it with breathable silicone plug;

2. After the outer wall of the strain tube is disinfected, in a sterile environment, use a small grinding wheel to draw a circle at 1/2 of the tube body, and then break it;

3. Absorb about 0.5mL of liquid culture medium into the strain tube, blow gently, fully dissolve and mix well;

4. Suck out all the dissolved solution of the bacterial powder and return it to the test tube, and plug the silicone plug;

5. Place the test tube in an incubator and culture under the above specified conditions. The culture solution is obviously turbid or precipitated, and the activation is completed.