Streptococcus thermophilus|BNCC186558 |BNCC

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Streptococcus thermophilus

Culture medium	MRS medium (MRS): peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.
Subculture procedure	(1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions	37 ℃;2-3 days; Anaerobic;
Storage conditions	2-8 ℃
morphology	MRS liquid culture bacterial fluid is turbid, there is a large amount of precipitation at the bottom of the test tube, and the growth of plate strains is weak. It is recommended to use liquid culture.
application	Detection of penicillin in milk; used in the production of probiotic health food. GB/T 4789.27-2008 Test for Antibiotic Residues in Fresh Milk
Sharing mode	Public welfare sharing

Streptococcus thermophilus

Storage conditions : 2~8 ℃

No. 186558

Product format : freeze dried,200ul

Validity period: 6 years

Biosafety level : 1

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. The freeze dried culture shall be used up once and shall not be retained. Handling instructions of suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions :37 ℃, anaerobic, MRS medium, 18-24h. MRS medium: peptone 10.0g, beef paste 10.0g, yeast powder 4.0g, glucose 20.0g, magnesium sulfate 0.2g, sodium acetate 5.0g, triammonium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.04g, Tween 80 1.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L. pH 5.7±0.2. Sterilization at 121 ℃ for 15min.

Recovery steps:

(1) Prepare a φ18mm sterile test tube of 25ml liquid medium; Silica gel plug is used for the liquid test tube;

(2) Sterilize the ampoule, use a small grinding wheel to make a circle at 1/2 of the tube body under strict aseptic conditions,, and then break it

(3) Draw 0.5ml of liquid growth medium into a freeze dried ampoule, make it full dissolved;

(4) Transfer all the solution to the liquid test tube, plug the tube with silica gel.

(5) Place the liquid test tube in incubator under the above growth conditions for cultivation, The culture solution is obviously turbid or precipitated, and this indicates the bacterial grows well.