False Armillaria (Armillaria, Armillaria, Hazel Mushroom)|BNCC143145 |BNCC

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False Armillaria (Armillaria, Armillaria, Hazel Mushroom)

Culture medium	Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L.
Subculture procedure	(1) Prepare 1-2 pieces of the plate; (2) sterilize the surface of test tube for the agar slant, open it in the biosafety cabinet; (3) cut into the pieces with aseptic inoculation hoe and inoculation shovel (see attached page). the size of square pieces is 0.5 × 0.5cm2; (4) lay flat the small pieces to the center of the agar plate; (5)put the plates under the above culture conditions, and the strains can be used when they grow.
Growth conditions	28 ℃; 5-7 days; Aerobic;
Storage conditions	2~8 ℃
morphology	Small filamentous fungi, with obvious colonies on comprehensive PDA medium, white hyphae at the initial stage, dark brown at the later stage, and born in the medium Long vacation root.
application	Production of Gastrodia elata
Sharing mode	Public welfare sharing

1. Description

1. Name: Armillaria mellea

2. BNCC No.: 143145

3. Biosafety level: 4

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C.

3. Growth Conditions:

1. Comprehensive PDA agar: potato extract 1.0L, glucose 20.0g ,KH₂PO₄3.0g ,MgSO₄.7H₂O1.5g, vitamin B1 trace g, agar 15.0g ,pH 6.0

2. Atmosphere: aerobic

3. Temperature: 25 ℃

4. Notes:

1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization.