BeNa Culture Collection
Subculture procedure | 1. Detection experiment: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 3. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 54 ℃ for 30s, and the second to third steps are cycled 40 times. 4. Fluorescence channel: FAM; |
Storage conditions | -20 ℃; protected from light |
Safety level | 1 |
application | Suitable for nucleic acid detection of human parainfluenza virus type 2 |
Sharing mode | Public welfare sharing |