Aspergillus terreus nucleic acid detection kit (fluorescent PCR method)|BNCC372817 |BNCC

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Aspergillus terreus nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Potato Impregnation Powder: 10.0, Glucose: 20.0,KH2PO4:3.0,MgSO4 · 7H2O:1.5, Thiamine: 0.008, Agar: 15.0,pH:6.0±0.2(25 ℃)
Subculture procedure	1. Experimental preparation: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Sample processing: After the positive quality control is dissolved, it is then placed on ice for later use; 3. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template and 8 μL of sterile ultrapure water, mix well and centrifuge for a short time; 4. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 30s, and the second to third steps are cycled 40 times. 5. Fluorescence channel: FAM;
Growth conditions	28 ℃;5-7 days; aerobic
Storage conditions	-20 ℃, dark and refrigerated
Safety level	1
application	For rapid detection of Aspergillus terreus
Sharing mode	Public welfare sharing