Vibrio fluvius nucleic acid detection kit (fluorescent PCR method)|BNCC373568 |BNCC

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Vibrio fluvialis nucleic acid detection kit (Fluorescent PCR method)

Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 60 ℃ for 40s, and the second to third steps are cycled 40 times; Fluorescence Channel: HEX.
Storage conditions	-20 ℃; protected from light
Safety level	2
Separation substrate	Human faeces, Bangladesh
application	Suitable for vibrio fluvius nucleic acid detection
Sharing mode	Public welfare sharing