Actinobacillus murinus nucleic acid detection kit (fluorescent PCR method)|BNCC372278 |BNCC

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Actinobacillus muris nucleic acid detection kit (Fluorescence PCR method)

Culture medium	Special peptone: 23.0, soluble starch: 1.0, sodium chloride: 5.0, agar: 10.0, defibrated sheep blood: 5% (added at 50 ℃),pH:7.3±0.2(25 ℃)
Subculture procedure	1. Detection experiment: dissolve the components in the kit on ice, mix well, centrifuge for a short time and put them on ice for later use; 2. Reagent preparation: reaction system (25 μL), take 15 μL of reaction solution, add 2 μL of template, 8 μL of DEPC-treated water, mix well, and centrifuge briefly; 3. Reaction conditions: the first step is 95 ℃ for 1min, the second step is 95 ℃ for 10s, the third step is 58 ℃ for 30s, and the second to third steps are cycled 40 times. 4. Fluorescence channel: FAM;
Growth conditions	culture temperature 37 ℃; culture time 24 hours; gas environment aerobic;
Storage conditions	-20 ℃, dark and refrigerated
Safety level	1
application	Rapid Detection of Actinobacillus roviticus
Sharing mode	Public welfare sharing