BeNa Culture Collection
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| Culture medium | Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃) |
| Subculture procedure | Before use, place the quality control sample at room temperature for 30 min, then open it in the biological safety cabinet, immediately add 5mL sterile water or sterile normal saline to redissolve the freeze-dried quality control sample, and the sample suspension will be obtained after fully mixing, with the concentration ≤ 200CFU/mL. |
| Growth conditions | Culture temperature 37 ℃; Culture time 18-24 hours; Aerobic atmosphere; |
| Storage conditions | -20 ℃ |
| Safety level | 0 |
| application | It is applicable to the detection of the total number of colonies, quality control and personnel assessment in the standard of GB 17378.7-2007 marine monitoring specification-Part 7: ecological investigation and biological monitoring of offshore pollution. |
| Sharing mode | Public welfare sharing |
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