Human parainfluenza virus (type I, II, III) typing detection kit (multiplex fluorescent PCR method)|BNCC378769 |BNCC

Essential Information Related Products

Human Parainfluenza Virus(I, II, III) Typing detection kit (Fluorescent PCR method)

Subculture procedure	Experimental preparation: dissolve the various components in the kit on ice before use, mix slightly and centrifuge for a short time, and place them on ice for later use; Experimental reaction system (25 μL):qPCR mixed reaction solution 15 μL, template 2-10 μL, and the rest supplemented with water; Experimental reaction conditions: the first step is 96 ℃ for 10min, the second step is 95 ℃ for 10s, the third step is 57 ℃ for 40s, and the second to third steps are cycled 40 times;
Growth conditions	culture temperature 37 ℃; culture time 72 hours; gas environment anaerobic;
Storage conditions	-20 ℃; protected from light
Safety level	2
application	Suitable for human parainfluenza virus (I, II, III) type detection
Sharing mode	Public welfare sharing