Standard substance for plate count of Listeria monocytogenes in food|BNCC384092 |BNCC

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Standard substance for the plate count of Listeria monocytogenes in food

Culture medium	Peptone: 10.0, beef powder: 3.0, sodium chloride: 5.0, agar: 15.0,pH: 7.3±0.1(25 ℃)
Subculture procedure	Take out the quality control sample from the refrigerator at -20 ℃, place it at room temperature for balance for 30 min, then open it in the biosafety cabinet, immediately add 5mL of sterile water or sterile physiological saline to redissolve the freeze-dried quality control substance, and prepare the sample suspension after fully mixing, which is the quality control sample with a concentration of 3000 ~ 9000CFU/mL.
Growth conditions	37°C, aerobic, 18-24h;
Storage conditions	-20 ℃, dark and refrigerated; ice bag transportation
Safety level	2
application	GB 4789.30-2016 National Food Safety Standard Food Microbiological Examination of Listeria monocytogenes Examination, Qualitative Examination of Listeria monocytogenes in Simulated Milk Powder Matrix Quality Control and Personnel Assessment (Positive)
Sharing mode	Public welfare sharing