BeNa Culture Collection
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| Culture medium | YM medium (English name: YM): yeast extract 3.0g, malt extract 3.0g, glucose 10.0g, peptone 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 6.2±0.2. Sterilization at 121 ℃ for 15min. |
| Subculture procedure | (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry; |
| Growth conditions | 30 ℃;24-48h; Aerobic; |
| Storage conditions | -80 ℃ |
| morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, milky white, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G + (blue-purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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