Quantitative strain of Escherichia coli|BNCC356331 |BNCC

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Escherichia coli

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Growth conditions	37 ℃;18-24h; Aerobic;
Growth characteristics	G-bacilli, lysine decarboxylase, ornithine decarboxylase positive, arginine dihydrolase negative, urease, phenylalanine deaminase negative, IMViC test: +, +,-,-;MUG positive, cellobisaccharide, side calendolol negative, glucose ammonium positive; no H2S, lactose fermentation acid production gas. Facultative anaerobic, optimum pH7.4 ~ 7.6.
Storage conditions	-20 ℃
application	Research; Analytical testing. Bacteria for research and quality control, "GB 4789.2-2010 colony count determination", "GB 4789.3-2010 coliform count", research; Analysis and testing. "GB/T 4789.32-2002 Coliform Rapid Test" and "GB 4789.39-2013 Fecal Coliform Count" Positive Control Strain, Quality Control Strain of Chinese Pharmacopoeia, 2015 Edition.
Sharing mode	Public welfare sharing