BeNa Culture Collection

Quantitative strain of Escherichia coli-BNCC
Quantitative strain of Escherichia coli-BNCC
Quantitative strain of Escherichia coli-BNCC
  • BNCC
  • Quantitative strain of Escherichia coli-BNCC
  • Quantitative strain of Escherichia coli-BNCC
  • Quantitative strain of Escherichia coli-BNCC

Quantitative strain of Escherichia coli

  • Price: $143
  • number:BNCC354963
  • Packing:penicillin bottle;(1.0±0.2)× 10 5CFU/bottle
  • Form:
    Pale yellow, raised, moist, bright, G-bacilli
Essential Information Certificate Related Products
Quantitative strain of Escherichia coli
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure (1) According to the culture conditions, the bacteria are first cultured into a bacterial suspension; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration of the bacterial suspension according to the standard formula; (3) The initial concentration multiplied by the survival rate is the actual concentration after freeze-drying; (4) Dilute the bacterial suspension according to the actual concentration after freeze-drying to obtain the target concentration, and then subpack and freeze-dry;
Growth conditions 37 ℃, aerobic, 18-24h
Storage conditions -20 ℃
morphology Pale yellow, raised, moist, bright, G-bacilli
Separation substrate Clinical isolates
application Sensitive test; Antibacterial test; Food testing; Cosmetic testing; Culture control; Quality control strain; API verification bacteria, GB 4789.38-2012 Escherichia coli count positive control strain.
Sharing mode Public welfare sharing

Escherichia coli

Strain number:BNCC 354963 / ATCC 25922

Name: Escherichia coli Escherichia

Product format: quantitative bacteria tablets           Number of viable bacteria: ( 1.0±0.2)×10^5CFU/bottle

Product batch: 220318                                             Valid until: 2024.03. 17

Inspection date: 2022.03.19                                      Sampling ratio: ≥2%

Report number: RS22354963-1                                  Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Item Inspection description results
stain microscopy gram negative and positive, cell morphology observed under microscope G-bacilli
colony morphology observation of single colony morphology pale yellow, raised, moist, bright
Purity non-selective medium culture, colony observation and microscopic examination No contaminated bacterial
Reference value of live bacteria Non-selective medium plate, viable count 1.0 × 10^5CFU/bottle
Phenotypic and Genotypic Analysis phenotypic testing, gene sequencing qualified
test conclusion After the BNCC quality control inspection system quality inspection, the product is qualified

Product list: One bottle of 1.0 × 10^5CFU quantitative strain, one bottle of 1mL dissolved solution and one copy of strain certificate;

Shelf life: unopened, below -20 ℃, 24 months;

Usage:

1. Open the strain and dissolved solution according to the arrow of Xilin bottle cap;

2. Pour the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve;

3. After full dissolution, it is (1.7±0.5)×10^4CFU/mL, can be absorbed in proportion according to the test requirements;

4. Please use up immediately after opening, and do not store it;

Notes:

① This product is a quantitative strain. improper storage conditions and usage methods will affect the accuracy of the number of strains, so please strictly follow the above storage conditions and methods.

② Used bacteria-carrying consumables, please autoclave and discard them.

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