BeNa Culture Collection
Culture medium | Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores. |
Growth conditions | 37 ℃, aerobic |
Storage conditions | -80 ℃ |
Separation substrate | Blood culture |
Sharing mode | Public welfare sharing |
Pseudomonas aeruginosa
No. :353546 / ATCC 27853
Product format: quantitative bacteria tablets
Number of live bacteria : 0.5-1.0x10 3CFU/bottle
Product batch : 200511
Valid until : 2021.5.11
Date of inspection : 2020.5.12
Sampling ratio : ≥2%
Report number : RS19353546-3
Biosafety level : 2 , handle in safety cabinet
item | description | test results |
stain microscopy | gram-negative and positive, cell morphology observed under microscope | G-bacilli |
colony morphology | observation of single colony morphology | light pink, translucent, producing pyogenin |
purity | non-selective medium culture, colony observation and microscopic examination | No bacteria |
Reference value of live bacteria | non-selective medium plate, live bacteria count | 610CFU/bottle |
phenotype and genotype analysis | phenotypic testing, gene sequencing | qualified |
test conclusion | after quality inspection by BNCC quality control testing system, the product is qualified |
Product list :0.5-1.0x10 3CFU quantitative strain 1 bottle, 1mL dissolved solution 1 bottle, strain certificate 1 copy;
Validity period: unopened, below -20 ℃, 12 months;
Handling procedure:
1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 0.5-1.0x10 3cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.
Notes:
1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization