Escherichia coli (Migula) Castellani and Chalmers|BNCC353545 |BNCC

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Escherichia coli (Migula) Castellani and Chalmers

Culture medium	Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (without liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	1. According to the arrow on penicillin bottle cap, open the strain and dissolved liquid; 2. transfer the bacteria tablets into the dissolved liquid, cover the rubber stopper, shake and dissolve; 3. After fully dissolved, it is 300 ~ 500CFU/ml, and absorbed in proportion according to the requirements; 4. Please use it up immediately after opening and do not store it;
Growth conditions	37 ℃, aerobic, 18-24h
Storage conditions	-80 ℃
morphology	The colony diameter is 1-2mm, round, with irregular edges, yellowish on the front, convex in the middle, smooth and bright surface, moist texture, easy to stir up, G-(red), bacilli, purity: pure
Separation substrate	Clinical isolates
application	Sensitive test; Antibacterial test; Food testing; Cosmetic testing; Culture control; Quality control strain; API verification bacteria, GB 4789.38-2012 Escherichia coli count positive control strain.
Sharing mode	Public welfare sharing

Escherichia coli

No.: 353545 / ATCC 25922

Product format: quantitative bacteria tablets

Number of live bacteria : 100 ~ 500CFU/bottle

Product batch : 200711

Valid until : 2021.7.11

Date of inspection : 2020.7.12

Sampling ratio : ≥2%

Report number : RS20353545-1

Biosafety level : 1 , handle in ultra-clean table or safety cabinet

item	description	test results
staining microscopy	gram-negative and positive, cell morphology observed under microscope	G-bacilli, in a single rod shape
colony morphology	observation of single colony morphology	milky white, raised, bright
purity	non-selective medium culture, colony observation and microscopic examination	No bacteria
Reference value of live bacteria	non-selective medium plate, live bacteria count	350CFU/bottle
phenotype and genotype analysis	phenotypic testing, gene sequencing	qualified
test conclusion	after quality inspection by BNCC quality control testing system, the product is qualified

Product list: 1 bottle of 100 ~ 500CFU quantitative strain, 1 bottle of 1mL dissolved solution and 1 copy of strain certificate;

Validity period : unopened, below -20 ℃, 12 months;

Handling procedure:

1 Open the penicillin-bottle according to the arrow indication on the vial cap;
2 Transfer the strain tablets into the vial of solution, capped with rubber plug, and shake to dissolve
3 It will be 100-500cfu/ml after full dissolved, and use appropriate amount according to the operation demand
4 The bacterial tablets shall be used up once and shall not be retained once the penicillin bottled is opened.

Notes:

1 This product is a quantitative strain. Improper storage conditions and handling procedure will affect the accuracy of the strain quantities. Therefore, please operate in strict accordance with the above mentioned instructions.
2 Waste generated from the handling process should be discarded after high-pressure sterilization