BeNa Culture Collection
Culture medium | YPDA medium (YPDA): yeast extract 10.0g peptone 20.0g glucose 20.0g adenine sulfate 0.03g agar 20.0g distilled water 1.0L. 116 ℃,30min sterilization. |
Subculture procedure | (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow. |
Growth conditions | 30 ℃;24-48h; Aerobic; |
Storage conditions | 2-8 ℃ |
Separation substrate | Not available |
Sharing mode | Public welfare sharing |