BeNa Culture Collection
Culture medium | Comprehensive PDA agar (CPDA): potato boil 1.0L, glucose 20.0g,KH2PO4 3.0g,MgSO4 · 7H2O 1.5g, vitamin B1 trace, agar 20.0g,pH 6.0±0.2. Sterilization at 121 ℃ for 15min. Potato boiling liquid: weigh 200g of peeled potato pieces, boil in boiling water for 30min, and collect the filtrate to a constant volume of 1.0L. |
Subculture procedure | Small filamentous fungi, the colony is obvious on the comprehensive PDA medium, the hyphae are white in the early stage, and gradually become yellow, and produce in the later stage Yellow spores, light yellow on the back of the medium. |
Growth conditions | 28 ℃;3-5 days; Aerobic; |
Storage conditions | 2-8 ℃ |
morphology | Small filamentous fungi have obvious colonies on the comprehensive PDA medium. The hyphae are white in the early stage and gradually turn yellow. The yellow spores are produced in the later stage, and the back of the medium is light yellow. |
application | Soy sauce producing bacteria |
Sharing mode | Public welfare sharing |
1.Description
1. Name: AspergillussojaeSakaguchietYamada
2. BNCC No.: 335940
3. Biosafety level: 4
2. Storage conditions:
Storage of freezed dried ampoule and agar slant at 2°C to 8°C
3. Growth Conditions
1. Comprehensive PDA:20% potato juice 1L, glucose 20g ,KH2PO4 3g, MgSO4.7H2O 1.5g, thiamine trace, agar 15g,pH natural.
2. Atmosphere: aerobic
3, Temperature: 25-28 ℃
4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.
2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.
3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.
4.Waste generated from the handling process should be discarded after high-pressure sterilization