BeNa Culture Collection

Escherichia coli (Migula) Castellani and Chalmers-BNCC
Escherichia coli (Migula) Castellani and Chalmers-BNCC
Escherichia coli (Migula) Castellani and Chalmers-BNCC
  • BNCC
  • Escherichia coli (Migula) Castellani and Chalmers-BNCC
  • Escherichia coli (Migula) Castellani and Chalmers-BNCC
  • Escherichia coli (Migula) Castellani and Chalmers-BNCC

Escherichia coli (Migula) Castellani and Chalmers

  • Price: $ 343
  • number:BNCC276498
  • Form:
    The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white front, flat shape, smooth and bright surface, moist texture, easy to stir up, G-(red), bacilli, pure
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Escherichia coli (Migula) Castellani and Chalmers
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid  medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 37 ℃,18-24h, aerobic
Storage conditions 2-8 ℃
morphology The colony diameter is 1-2mm, round, with neat edges, opaque, gray-white front, flat shape, smooth and bright surface, moist texture, easy to stir up, G-(red), bacilli, pure
Sharing mode Public welfare sharing

C8-D1A mouse cerebellar astrocytes

Adherent, fibroblast-like cells

Culture:37 ℃,5% co 2 ;CM1-1 culture solution

No.: 274179

Product format:  2ml frozen vial x 2, or T25 flask x 1

Biosafety  level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the cells are well. For the frozen cells,  it shall be stored in the refrigerator at -80 ℃ upon arrival. If they are not used for a long time, they shall be transferred to liquid nitrogen for storage overnight.  Recovered cells in T25 culture flask, upon receipt, put the culture flask in the incubator for 4h, and then carry out handling procedure. During recovery, each vial shall be used up once and shall not be retained. After recovery, the cells can be passed on to the next generation and can be used normally. Please operate in strict accordance with this instruction, otherwise the replacement of cells are not be available in case of loss of cell viability.

Growth conditions:37 ℃,5% CO2,CM1-1 culture solution. CM1-1 culture solution: 90% DMEM-H + 10% FBS. DMEM-H:DMEM high sugar culture solution, containing glutamine and sodium pyruvate.

Recovery steps:
(1)Prepare a new 100mm culture dish containing 12ml of the above culture medium;
remove the frozen vial from liquid nitrogen or refrigerator at -80℃,  thaw the vial in  37°C water bath for 1-2 minutes. Transfer the vial to biosafety cabinet for culture as soon as the contents are completely thawed.
(2)Draw the solution with a sterile pipette, drip into a new culture dish, and mix it evenly by shaking clockwise;
(3)Put it into incubator (37℃,5%CO2), change the media overnight, and it will grow up in 2-4 days.

Subculture/ cryopreservation:  remove old medium, and rinse twice with PBS, add 2ml of 0.25%Trypsin+0.02%EDTA, do not shake the culture dish during the period. Observed under the microscope, remove most of the trypsin(with 0.5ml left) when the cells detach. transfer it to the incubator for digestion about 1minute, and take it out.

(1)Passage: terminate digestion with 6ml of CM1-1 culture media, aspirate and dispense  into 3~6 culture dishes.

(2) Cryopreservation: terminate digestion with 3ml of cryopreservation solution (90%FBS+10%DMSO), aspirate and dispense into 3 frozen vials, and cryopreserve at -80 ℃ with a programmed cooling box.

Recovery record:  According to the recovery instructions, the results of the cell recovery are reported as follows:

item quality standard recovery record
resurrection adherence is observed in 18 hours, the cell adherence rate ≥ 80.0% in 100 hours good viability, no abnormal cell morphology, qualified
cell morphology adherent, fibroblast-like cells CM1-1 culture medium, adherent, fibroblast-like cells, long spindle
attached
Conclusion good resurrection, no abnormal cell morphology, qualified
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