BeNa Culture Collection

Bacillus circulans-BNCC
  • BNCC
  • Bacillus circulans-BNCC

Bacillus circulans

  • Price: $ 143
  • number:BNCC186120
  • Form:
    Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Uplift: Flat Surface: Smooth Gray Texture: Wet
Standard strain Quantitative strain DNA extraction
Package:
  • freeze dried
    Freeze-dried: lyophilized strain, freeze dried vial, used up at one time and not be retained
  • agar slant
    Agar slant: growing culture, ready to use, Short term preservation, convenient access
Essential Information Certificate Related Products
Bacillus circulans
Culture medium Nutritional gravy medium (NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mg MnSO4 · H2O when culturing Bacillus. it is beneficial to produce spores.
Subculture procedure (1) Prepare a test tube containing 5~10mL of liquid medium and 2 plates; (2) Open it in the safety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps; (3) draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly; (4) inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates; ⑤ Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.
Growth conditions 30 ℃;18-24h; Aerobic;
Storage conditions 2-8 ℃
morphology Size: 1-2mm Shape: Round Edge: Irregular Transparency: Opaque Color: off-white Uplift: Flat Surface: Smooth Gray Texture: Wet
application Antibacterial activity test of enzyme preparations for food processing. Microbial strains for fertilizer production
Sharing mode Public welfare sharing

Bacillus circulans

Storage conditions: 2~8 ℃

No. 186120

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety  level: 2, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar plate is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions: 30℃, aerobic, nutrient agar medium. 18-24h, nutrient agar medium: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L, pH 7.0. Sterilize at 121°C for 15min.

Recovery steps:

(1)Prepare a test tube containing 5-10mL of liquid culture medium and 2 plates;

(2) Open the ampoule in the biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

(3) Draw 0.5mL of liquid culture medium into a freeze dried ampoule, fully rehydrate and transfer the solution to the liquid test tube, mix evenly;

(4) Inoculate a plate with 0.2mL of suspension liquid, repeat the step to obtain two plates;

(5) Put all the liquid test tubes and plates under the above culture conditions for cultivation, and the strains can be used when they grow.

Recovery record:  According to the recovery instructions, the results of the recovery are reported as follows:

                                                       

 

Item test results
viability good viability,in 20h  the liquid medium is turbid, and the plate bacteria layer is obvious; The bacterial liquid is marked on the plate, and the colony is typical
colony morphology: (above)

size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque

color: off-white ; Uplift: flat ; Surface: smooth and dark ; Texture: moist

Conclusion: good viability, no abnormal colony morphology, qualified

 

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